International Journal of PharmTech Research, Vol 9. No. 7 (2016)

Isolation of Angiotensin Converting Enzyme (ACE) Inhibitory Activity Quercetin from Peperomia pellucida

Agus Kurniawan1,3, Fadlina Chany Saputri2, Rissyelly1, Islamudin Ahmad1,
and Abdul Mun’im1*

1Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy,
Universitas Indonesia, Depok, 16424 Indonesia
2Department of Pharmacology, Faculty of Pharmacy, Universitas Indonesia, Depok
16424, Indonesia
3Department of Pharmacy, Faculty of Medicine and Health, University of Gunadarma,
Depok 16424, Indonesia


Peperomia pellucida (L.) Kunth. (Piperaceae) is empirically used in traditional medicine to control blood pressure. The objective of this study was to isolate ACE inhibitor from aerial part of the plant. Using chromatography technique was isolated flavonoid as ACE inhibitor. The chemical structure was determined as 3’,4’, Dihydroxy-3-5-dimethoxy flavone-7-O-β-rhamnose based on the spectral data (UV, IR, LC-MS, 1H-NMR, 13C-NMR, and 2DNMR) and compared with that reference. In vitro assay of ACE inhibitory activity that showed IC50 of the compound was 7.72 μg/mL. The result of kinetic suggested that the compound inhibit the enzyme activity by competing with the substrate for the active site. The results supported the traditional use of the plant antihypertensive.

Key words : Peperomia pellucida, ACE, isolation, antihypertensive, flavonoid.

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