Metabolite profiling analysis of conventional and non-conventional extraction methods on a secondary metabolite from Peperomia pellucida (L.) Kunth using UPLC- QToF-MS/MS System
Islamudin Ahmad1,2, Kamarza Mulia3, Arry Yanuar2, Abdul Mun’im2
1Department of Pharmaceutical Sciences, Faculty of Pharmacy, Mulawarman University, Samarinda, East Kalimantan, 75119, Indonesia
2Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, 16424, Indonesia
3Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java, 16424, Indonesia
Correspondences: Islamudin Ahmad (firstname.lastname@example.org) and Abdul Mun’im (email@example.com)
Objective: The purpose of this study was to know the difference of extraction method (both conventional and non-conventional) based on metabolite profile using UPLC-QToF-MS/MS system from Peperomia pellucida (L.) Kunth. Methods: Dried samples were extracted using the conventional maceration method and the optimum of ionic liquid-based microwave-assisted extraction (IL-MAE) methods. Metabolite profiling was performed using UPLC-QToF-MS/MS system with some modification adjusting to the instrument condition and data was analyzed using Masslynx 4.1 software. Results: based on the results, showed the difference of profiling metabolite from both conventional and non-conventional extraction methods that was extraction method using organic solvent and ionic liquid solvent ([BMIM]BF4 at the optimum condition. The extract obtained using ILMAE method had a peak depth with a well-separated Rt (retention time) value ranging from 0.5 to 7.5 minutes which means that the extracted compound was from polar to nonpolar properties. The extract obtained using maceration method, the peak spread on a separate Rt ranges from 2.5 to 7.5 minutes. Also, both extracts obtained have different area under curve (AUC) values with amount total of 22285 (IL-MAE) and 12679 (maceration), respectively, and showed the IL-MAE twice as large as that of the maceration. Conclusion: based on the results, conventional and non-conventional extraction method showed differences in metabolite profiling based on Rt value and mass spectrum m/z of each peak.
Keywords: Peperomia pellucida (L) Kunth, maceration, ionic liquid-based microwave-assisted extraction, metabolite profiling, hyphenated system