3rd MIPS-2017

This article already presented in 3rd Makassar International Symposium of Pharmaceutical Sciences, November 3rd – November 4th 2017, Four Point of Sheraton Hotel, Makassar, South Sulawesi, Indonesia

Pharmacognostical characteristic, metabolite profiling, and angiotensin converting enzyme inhibitory activity evaluation of the methanol extract of Peperomia pellucida (L.) Kunth herbs

Islamudin Ahmad1,2, Neneng Siti Silfi Ambarwati2,3, Desy Rasta Waty2,4, Fadlina Chany Saputri5, Abdul Mun’im2

1Laboratory of Pharmaceutical Research and Development of FARMAKA TROPIS, Faculty of Pharmacy, Mulawarman University, Samarinda, East Kalimantan, Indonesia

2Laboratory of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, Indonesia

3Faculty of Engineering, Universitas Negeri Jakarta, East Jakarta, Indonesia

4Directorate of Food Product Standardization, Indonesia National Agency of Drug and Food Control, Jakarta, Indonesia

5Laboratory of Pharmacology, Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, Indonesia

Abstract

Background: Peperomia pellucida (L.) Kunth is a herb can be found in various shaded and damp habitats in tropical and subtropical climate. This plant is traditionally used as a medicine in Indonesia such as dizziness, headache, fever, gout, and hypertension. Objective: This study aimed to determine of pharmacognostic characteristic and angiotensin converting enzyme (ACE) inhibitory activity evaluation of P. pellucida methanol extract. Method: Extraction process was performed using gradual maceration (n-hexane and methanol). Pharmacognostic characteristic was determined using some parameters including metabolite profiling, total phenolic and flavonoid content, and physicochemical characteristic. Also, ACE inhibitory activity was evaluated using KIT ACE WST1 and microplate reader 96 wells. Results: In this work was obtained the yield of methanol extract (10.4%w/w). Metabolite profiling on methanol extract was showed a peak depth with a well-separated Rt (retention time) value ranging from 3.5 to 8.0 minutes which means that the extracted compound was from polar to nonpolar properties. Phenolic content and flavonoid content in methanol extract were 4,33% (as gallic acid) and 2,53% (as quercetin), respectively. Physicochemical properties were a specific odor, a distinctive taste, a greenish brown, the pH of 6.88, the water content of 3.62%v/w, the total ash content of 1.68%, the acid insoluble ash content of 0.05%, the ethanol soluble extractive of 82.46%, and the water soluble extractive of 16.74%. Whereas, ACE inhibitory activity (IC50) was 22.80 µg/ml. Conclusion: based on the results studies, the methanol extract had a potential as ACE inhibitory activity with the specific pharmacognostic characteristic.

Keyword: Peperomia pellucida (L) Kunth, methanol extract, pharmacognostic characteristic, ACE inhibitory activity.

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