Optimization of ionic liquid-based microwave-assisted extraction of polyphenolic content from Peperomia pellucida (L) kunth using response surface methodology
Islamudin Ahmad, Arry Yanuar, Kamarza Mulia, Abdul Mun’im
Objective: To optimize the ionic liquid based microwave-assisted extraction (IL-MAE) of polyphenolic content fromPeperomia pellucida (L) Kunth.
Methods: The IL-MAE factors as experimental design parameters, including microwave power, extraction time, ionic liquid concentration, and liquid–solid ratio had been involved. Response surface methodology and Box–Behnken design were used to obtain predictive model (multivariate quadratic regression equation) and optimization of the
extraction process. The response surface was analyzed by using the yields of total polyphenolic content as response value.
Results: Based on the results was obtained the optimum extraction condition, including microwave power of 30% Watts, extraction time of 18.5 min, the ionic liquid concentration of 0.79 mol/L, and the liquid–solid ratio of 10.72 mL/g 1-Buthyl-3-methylimidazolium tetrafluoroborate ([bmim]BF4) as a solvent was selected. The regression model was obtained to predicts the yields fromPeperomia pellucida: Y = 30.250– 1.356X1 + 2.655X2 + 2.252X3 – 0.565X4 + 0.990 X1X3 – 8.172 X1X4 – 3.439 X3X4 – 4.178 X1 2 – 3.210 X3 2 – 6.786 X4 2 – 7.290 X1 2X3 + 5.575 X1X3 2 – 4.843 X3 2X4 with R2 = 0.82519. Scale-up confirmation test was obtained the maximum yields of total polyphenolics content with the amount of 31.172 5mg GAE/g.
Conclusions: The IL-MAE method produced a higher extraction polyphenolic and performed rapidly, easily and efficiently.
Keywords: Peperomia pellucida (L) Kunth, 1-buthyl-3-methylimidazolium tetrafluoroborate, Response surface methodology, Box-Behnken design, Polyphenolic content
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