International Journal of PharmTech Research, Vol. 9 No. 9 (2016)

THE EFFECT OF EXTRACTION METHODS OF BAWANG DAYAK (ELEUTHERINE PALMIFOLIA L. MERR) AGAINST TLC PROFILES AND SUNSCREEN ACTIVITIES

Islamudin Ahmada*, Muhammad Arifuddinb,  Laode Rijaia

aDepartement of Pharmaceutical Sciences, Pharmacy Faculty, Mulawarman University, Samarinda, East Kalimantan, Indonesia

bPharmaceutical Research & Development Laboratory of FARMAKA TROPIS, Pharmacy Faculty, Mulawarman University, Samarinda, East Kalimantan, Indonesia

*Corresponding author: islamudinhmad@farmasi.unmul.ac.id  Mobile: +6281342205060

 

ABSTRACT

A research has been conducted on the effect of extraction methods of Bawang Dayak (Eleutherine palmifolia (L.) Merr) against TLC profiles and sunscreen activity. The study aims  to determine the effect of extraction methods against yield, TLC profile, and sunscreen activities. The Sample was extracted using several extraction methods (maceration, reflux, and soxhlet) with different polarity solvent (n-hexane, ethyl acetate, ethanol, dan methanol) and  single solvent (methanol), respectively. Yield value obtained methanol extract (single solvent) are 8.027% maceration, 6.943% reflux, and 10.694% soxhlet. N-hexane extract are 1.572% maceration, 1.251% reflux, 2.146% reflux. Ethyl acetate extract were 2.141% maceration, 0.966% reflux 1.681% soxhlet. Ethanol extract are 3.537% maceration, 3.280% reflux, 3.974% soxhlet. Methanol extract are 1.999% maceration, 2.553% reflux, 2.682% soxhlet. Furthermore, the determination of the TLC profile from all methods using comparisons N-hexane: ethyl acetate among other; N-hexane extract was 6-7 spots (3:1), ethyl acetate extract was 9 spots (2:1), methanol extract (single solvent) was 8 spots (2:1). Determination TLC profile using comparisons chloroform:methanol eluent namely; ethanol extract was 9 spots (7:1), methanol extract was 5 spot (2:1), and methanol extract (single solvent) was 8 spots (5:1). Sunscreen activity for ethyl acetate extract (using soxhlet methods) categorized as sun block and other as extra protective.

Keywords : Eleutherine palmifolia (L.) Merr, extraction methods, TLC Profile, sunscreen activity

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